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contains a segment with Hoyt's mother attempting to intervene in Hoyt's relationship with newborn vampire Jessica. Hoyt's mother turns up at his workplace with Summer in tow (whom she believes Hoyt should be dating instead of a newborn vamp), along with the local school's guidance counselor. Hoyt says he has work to do and doesn't have time to talk, but the guidance counselor, acting as the "interventionist", stops Hoyt from leaving, parodying Jeff VanVonderen's traditional intervention opening lines ("I'm here for these folks who really love you like crazy, and want you to hear them out, and then you can say what you want to say"). The characters then read their letters out loud, all of which open with "Dear Hoyt" (similar to letters the families normally write to their loved ones as part of the interventions depicted in Intervention ).

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that first aired October 18, 2011, host Daniel Tosh turns his normal "Web Redemption" segment (where a person or group who appeared in a notorious or embarrassing online video are given a chance to explain themselves via interview/event recreation) into an "intervention" for a Wisconsin man named Tim, whose videotaped trip on shrooms turned into a YouTube sensation. Tosh reveals the redemption segment is really an "intervention" about 2/3 of the way through when he lures Tim into leaving the room with him on the pretense of going to see "a Lady Antebellum laser light show"; when they open the door to leave the interview room, Tim discovers they are in a small hotel conference room, in which Tim's "family and friends" are gathered all around. Tosh uses Jeff VanVonderen's trademark intro ("These people love you like crazy...") and introduces Tim's mom and dad, some other people "who are probably early for the next intervention", and a clown who's "addicted to smiles" that Tosh invited because "these things are always so depressing." Tim's father starts off the family portion of the intervention by reading his own letter; it begins with "we're here because we wanted a free trip to L.A." The interventionist quickly determines that Tim hasn't yet hit rock bottom and rescinds the offer of rehab until he does. Tosh and Tim are shown next attending a drug party, where they re-enact many of the crazy things Tim said and did in his notorious YouTube video, and a black screen with white text reveals that two minutes have passed since they began their drug party. As Tosh decides that Tim has now hit rock bottom (since they find the dead body of the clown from the intervention beside their hotel bed), they head off together to the rehab center. But when " Amy Winehouse " answers the door at the "rehab center", the pair realize they must have overdosed (a subtle satire on one of Ken Seeley's assertions during Pre-Intervention talks, when he stresses that some addicts don't hit rock bottom until they're dead). As they enter Heaven, the screen fades to white, where black text (instead of the usual fade-to-black with white text summarizing the addict's time in treatment) says simply that "Six months later, they are still happily dead." [10]

Table 1

Baseline characteristics

-value denotes differences between those with and without AMI. angiotensin converting enzyme inhibitor or angiotensin receptor blockers, body mass index, coronary artery bypass graft, high-density lipoprotein, low-density lipoprotein, left ventricular ejection fraction, myocardial infarction.

Figure 1

Differential expression according to coronary artery disease status. (A,B) PC3 scores by disease classification in the two phases of transcriptome profiling. -values from ANOVA with three degrees of freedom for group effect, implying replicated differential expression between individuals presenting with AMI and those with NO CAD, CAD and OLD MI, which are not consistently divergent. (C,D) Volcano plots of significance against difference in expression in log2 units of SNM-normalized data with genes upregulated in patients with AMI to the left, and genes downregulated in AMI to the right. Colored transcripts are significant at  < 10 in phase 2. Almost all of these are differentially expressed in the same direction in phase 1, which, however, showed much less evidence for significant differential expression in the total sample (possibly reflecting lower technical quality, or unknown covariates biasing against the AMI association). (E) Volcano plot of differential expression between non-AMI individuals who have had an MI on follow-up (n = 11) and the remainder, all in phase 1.

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C,D. The effect of AMI is stronger in the replication phase, but the vast majority of the genes showing highly significant differential expression in either direction in the replication phase (red or blue spots highlighted in Figure 1 D) showed the same direction of effect in the discovery phase (Figure 1 C), indicating replication of the AMI effect. Remarkably, differential gene expression in 11 individuals who had an MI during the follow-up period for the discovery sample also tends to be in the same direction (Figure 1 E; insufficient sample size for replication phase). Furthermore, Kiliszek et al . [ 13 ] reported 24 genes that were differentially expressed in a set of 28 patients experiencing AMI relative to controls, as well as at 6 months follow-up, and although these genes are not the most significant in our list, PC1 for their 24 genes is significantly associated with AMI in our cohort (one-tailed t -test, P  = 5 × 10 -5 ) and corresponds very closely to the major component of AMI-associated gene expression in our study. This differential ranking of top genes is to be expected as the most significant transcripts in any particular sample will be influenced by sampling variance.

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